FastQCFastQC Report
Mon 9 Apr 2018
HYKJMAFXX_n02_grads_set5_healthy_rna.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYKJMAFXX_n02_grads_set5_healthy_rna.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39364776
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGAGAGCGTAGTCGAGATACCGCGAGAGCGTAGTCGAGATACCGCGAG2869870.7290451748029761No Hit
CGTGCGACGACGACGCTGTAGATGGGGCTGGGCCCGCATCATCAATGCGA2344240.5955171699694163No Hit
TCAAGCAGACCAAGGCCTACACCTACTTCAAGCCGAAATACATCTTCTAC2326200.5909343927169813No Hit
TTGAGCAGGTCGTACTCGCCGACCGCATAGACCAGCGAGGTGTCCTGGAA1511750.3840362256856231No Hit
CGTGCGACGACGACGCTGTAGAGAGCCTCATTCGACGAGTGGGACGGAGG1471720.3738672360284738No Hit
CGTGCGACGACGACGCTGTAGATTCTTCACGTAGCGCGACAGGCCGGACG1437840.36526055679829095No Hit
TCAAGAGCCAGGGTGTCGATGAAGGTGCGATGACGCTGCAGGCAAGCAAC1429840.36322828307215566No Hit
CGCTGACGTCGTAGCACACTCGGGTCAAGTGAAGAAGCGCATACGGTGGA1418770.360416124303616No Hit
TTAAGGGCTAGTCCCCGTTCGCTCGCCACTACTAAGGGAATCTCGGTTGA1376080.3495714036325267No Hit
TCGAGCGGCGGCAACACGACGCAATCGGTCGCGGGCGCGCTGCAGCAGCC1375720.3494799513148506No Hit
CGTGCGACGACGACGCTGTAGATGGTGATCCTGTGGTTCTTCCTGCTGAT1333800.33883083698990185No Hit
CGTGCGACGACGACGCTGTAGAACGGCATCATCACGGCAAGCGCTGCCAC1199010.30458956504668033No Hit
TCGAGCACGCCCTCGTTGACCGAAGTGGCGAGGAGGTCCCACAGCAGGAA1053430.26760726391533385No Hit
CGTGCGACGACGACGCTGTAGATCATGTACCCGCGCGACCAGTATCCCGA1013660.2575043231542839No Hit
TTGAGCACTTCGCGCACCCGATCGCTCTTGCTGGCGCCAACGAACAGCGC968920.24613883234087244No Hit
CGTGCGACGACGACGCTGTAGACCGGCAAGGGTCGTGCCGTTGCCGTATC931920.23673956635749688No Hit
TTAAGCGAGCGGGCAACACGCGTCGGCTTCAGGATCAGATGGTGCCGGTG931310.23658460548587906No Hit
CGTGCGACGACGACGCTGTAGATGATCGTCGGCCCGTTGCTGATCGGCCT902440.22925063767668843No Hit
TCAAGCGCCTGCGGGGCTCCGCGGCGCGCGCGTCAGCAGCGCGAACACCG891940.2265832784111359No Hit
TTGAGTTCAGCGATCGTCTTGGCCAGGTTTTCCGGCTCGTTCACGTCGAG857110.21773526667597445No Hit
CGTGCGACGACGACGCTGTAGATGAGGCCAGTGCACAGGATATTTTTTTT804310.20432226008348175No Hit
CGTGCGACGACGACGCTGTAGACAACGCGGCGACCGCCGCGCAGGCTGAA757810.19250966905032052No Hit
TCAAGAACCAATTAACCAGAAATTATCTTGAAAACCTCTCCGGATCTACC754110.19156974245198294No Hit
CGTGCGACGACGACGCTGTAGAGCCTTTCTCACGAAGTCTGCCAAGCAGA746450.18962384035920843No Hit
TCAAGAAGCCGGATTGGATTCGCGTGCGCGCCGCCACCGGCAACTCGCGC736840.18718257154568846No Hit
TCAAGCGCAATGTGCGTGCATAGTCGCTGTGATTCCAATTGCCAACGATC735700.1868929725397142No Hit
TCAAGGGAGGGGGCGCCGATTGGGCGACGGTCGATGCAAATACGATCGAC730560.18558723667067228No Hit
TCAAGAGCGGCCTGGCCCGCGGGCAAGGCGAGATCGCTGGCAGCCTGGTC712500.1809993787339219No Hit
CGTGCGACGACGACGCTGTAGAGCGCGGCCTGTACGCCAGCCAGATGCTG695100.17657918337957773No Hit
CGTGCGACGACGACGCTGTAGAGCGGCTGTATCGTGGTGCCTGATGGCGG689980.17527852819485115No Hit
TCGAGCCGCGTCGAATAGCCGGGGATGTCGCCAAGCAGCAGGCCACTGGC682140.1732868999432386No Hit
TTGAGCTGCTTCAGGCGCTGGCGCTCCACGTCGTTGGTCGTCGCGGTGAT681040.173007462305895No Hit
TTGAGCTGCTCGGCCATGTCCTCCATGCCGGGCTGGTTGCCAAAGGGCGT570430.1449087376999173No Hit
CGTGCGACGACGACGCTGTAGAAGCTGGAGAAGCGCTTCGCGCAGGAGAT554380.14083148853685842No Hit
TCAAGCTCCTTCAGCGCTGCCTGGGTGAACTTCTCGCCGAGTGCCTTGGA553440.14059269637403754No Hit
CGTGCGACGACGACGCTGTAGAAGGGACCGTATGAGGGCCGGGCTACTGG543690.13811586277031018No Hit
TTGAGTAATCTGCAAGGACTCTTCTGGGTTTGCAAACCAGACTCCCCCCT537280.1364875034472443No Hit
TTGAGGCTGACATGGATCGTGTCCTGCGGCAGGCCCGCATGGCCGTAGAT519000.13184375798302525No Hit
TTGAGCACCTTGCTGGTCATCTCACCGATGACCTTGGCGCGCGGATCGAA517570.13148048905447854No Hit
TTGAGCTTGGACGTGCCATGGCCTTCGATCTTGGCGCCCAGCAGCGACAT506610.12869627404967326No Hit
CGTGCGACGACGACGCTGTAGATGCTCGCCGAGCTGCAGCAGCGCAACAA505200.1283380858054419No Hit
TTGAGCAGGTAGGCGGCTCCTAGCTCGGGGTAGTTCTTCGTCCATCCGTC498700.12668686340295698No Hit
TTGAGTGTGATCGCGCCCTGTGTGAAGTGGCCCACCTGAGCCACCGGGTG492720.12516773879267087No Hit
TTGAGTTGCGGCACAGCATCGGGAGCAGGGGTCTCGATGAGATCGACCTG487550.12385438189715597No Hit
CGTGCGACGACGACGCTGTAGAGAAGGCGCAGCCTGCGCTCGACCGCGGC478600.12158077566604215No Hit
CGTGCGACGACGACGCTGTAGATGCTGGAAGAGATCGGCAGCGCCGACAA477100.12119972434239178No Hit
CGTGCGACGACGACGCTGTAGAAGTAGCACTCGCCCTGGATTGCGCCGTA477080.12119464365807646No Hit
CGTGCGACGACGACGCTGTAGAGATCACCACCAAGACGGTCGATTCATTC476300.12099649696977825No Hit
TCGAGCTTCACCTACGCGCAGGCGACGTGGACGCAGGGGCTCGCCGACTG468970.11913442616820681No Hit
TTGAGCAATATTTTGACCGAATCAACCAGGTCTATGTTGTTCTGAAAGTG468130.11892103742696261No Hit
CGTGCGACGACGACGCTGTAGAAGGACGGCCTGCTCTACCGCAACAAGGT453160.115118145216932No Hit
CGTGCGACGACGACGCTGTAGAAAGCGCAGGTCGACCATCCGCTGCCGCC449160.11410200835386439No Hit
CGTGCGACGACGACGCTGTAGATGGCTCCAACCCTGGGGAGTGTACTTTC446860.11351772965760049No Hit
TCGAGGGTGAAGGCAACGACTATGTCGGCAAGGGCCTGTCGGGCGGCAAG442870.11250413313669053No Hit
CGTGCGACGACGACGCTGTAGAAGCACCGTATTCTAAAGCAAGCCCAGCC442620.1124406245827488No Hit
CGTGCGACGACGACGCTGTAGATTCTGGATATTAGACCTTTGTTGGATGT441850.11224501823660829No Hit
CGTGCGACGACGACGCTGTAGAGAGCTGGAACTGGTGGCCGCGCTGCACG435040.11051504522723564No Hit
CGTGCGACGACGACGCTGTAGATGTCGACCTCCGTCAATGAGCCGACCTT433840.11021020416831534No Hit
TCGAGGCGCCGAGCACGGCGACGAAGATCTGCGCCGTCCTCCGCTCGCCG432070.10976056360640792No Hit
TTAAGAATGTCATCGTAGATGACCTTCATTTCCCGTGTGGGCCATTCTGA430890.10946080323180297No Hit
CGTGCGACGACGACGCTGTAGATGACGGCGGCATCGGGGTCGGCGCGGTG428670.10889684727280044No Hit
TCGAGAACGACTGGCCCTGCGGCGTCGTCAGTTCGGGGTGGTCCCACACG428620.10888414556201209No Hit
CGTGCGACGACGACGCTGTAGATCCTGTGGGAGGAGTACATCGGCGCCGA427630.10863265168840285No Hit
TCGAGATCCCGGATGCGCGCTAAGCTCGCTCCGGGATGACGCGCTTTCGC423910.10768764440574996No Hit
TCAAGATGGATCAAGGACTTAAATCTAAGACCTGAACCATAAAAATTCTA423760.10764953927338491No Hit
CGTGCGACGACGACGCTGTAGACCGTCTTGCCGGATGCCCGCATCGTCAT423380.10755300627139348No Hit
TTAAGGAGGCTGGTATCAAATACAAAGTTGGTAAATTCCCATTTATTGCT423300.10753268353413215No Hit
CGTGCGACGACGACGCTGTAGAGCGCATCCTGGACGTGCTGCTGCCCGGC415100.1054496029648435No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG415080.10544452228052816No Hit
TTGAGGTGGCCGCGCAGCGACGCCTCGCCCTTGGTGTCGGTGCGCGACTT411000.10440806268019918No Hit
CGATACAGCTACTACTACTCTATGAAAAGAAAGGTTAAACTCTGTGAGTT410780.10435217515273046No Hit
CGTGCGACGACGACGCTGTAGACGACGCTGTAGAAGACGCGGTGGCGCAG405520.10301595517779652No Hit
TCGAGCTGTATCATGCCGTCGTGACCGGCGTCGCCGACGCAACGAAAACG398000.10110561787522937No Hit
CGTGCGACGACGACGCTGTAGATCGGCCAGAAGACGCTGCTGCGCCTCTC397400.10095319734576921No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGA7513100.0141.339721
GTGCGAC7520900.0141.160692
TGCGACG7706000.0137.646773
ACGACGA7985000.0134.081597
CGATACA2748000.0133.70871
GCGACGA8117950.0130.842964
GCGACTA1044500.0129.725392
CACGCAC1071900.0122.5845959
TACACGC1108950.0122.514437
CGCGACT1114050.0122.152391
ACACGCA1092350.0120.0260248
CTACACG1147150.0119.3922046
TGACGTC1042300.0117.228114
CTGACGT1061900.0115.127843
TTAAGCG158850.0114.653561
ACTACAC1202900.0113.133935
GTCGTAG1115600.0111.8516548
ATACAGC3349950.0110.1194153
CGTCGTA1145100.0108.9638447
TCGTAGC1164650.0107.388239
Produced by FastQC (version 0.11.2)