FastQCFastQC Report
Mon 9 Apr 2018
HYKJMAFXX_n02_grads_set5_healthy_dna.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYKJMAFXX_n02_grads_set5_healthy_dna.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42852829
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGCCTTACACTGACTTCCACAATGGTTGAACTAGTTTACAGTCCCACC3826620.8929678831705603No Hit
CGACTGTACGTCTACATCGTCACTGGCCATCAGAGAAATGCAAATCAAAA2184890.5098589873728057No Hit
CGACTGTACGTCTACATCGTCAGTGGCCATCAGAGAAATGCAAATCAAAA2155290.5029516254341108No Hit
CGCGTAGAGATATAGACGTAGAGAAGAGCAAACGCCTCCTGGCAGGCGGA1866460.4355511744627175No Hit
CGCGTAGAGATATAGACGTAGAGTGTGTTCAGGTGTGTGTGTGATGGGGC1627090.37969255191996776No Hit
TCAAGTTTTTGCATTTCCTTTTCATGTTCTACTTCGTGATTTCTTTTTTA1604200.3743510142585919No Hit
CGCGTAGAGATATAGACGTAGATACAGTGTTTATGTCAAGTGCAGCTATA1373740.32057160100211823No Hit
CGCGTAGAGATATAGACGTAGATTTGGTCTTTTCACATAGTCCCATATTT1352840.31569444341702624No Hit
TCAAGCCTCTCCCTGAGCCTGGGGACACAGGGACAGCATCAGAAATGGAT1335730.31170170818827386No Hit
TCAAGGAGTTCACTGTCTAACAGAAGAGACAGAGAAGACAACTAAGCAGC1128190.2632708332978437No Hit
CGCGTAGAGATATAGACGTAGACAGGCAGTCCTGGGCACAGCCTCCCATC1119540.26125229678535344No Hit
CGCGTAGAGATATAGACGTAGAGAATGTAAAGCCACCACACCACTAAGTC1090860.2545596231231315No Hit
TTGAGTGTGTTTTTGAAGGAGCATGTTTCAGTTCTTTGTTTTCTGTTTGC1035690.24168532723942218No Hit
CGCGTAGAGATATAGACGTAGACACACTGCGCTAGATACAGATACAGAGC992140.23152263763029507No Hit
CGCGTAGAGATATAGACGTAGAACATTCCCTTTGGTAGAGCAGGTTTGAA977320.22806428952450256No Hit
TTGAGACCAGGAGTTCCAGGCTGCAGTGAACTGTGATCACACCACTGAAC891040.20793026290049604No Hit
TCGAGAACTACGTGAAGAATGCAGAAGCCTCAGGAGCCGATGCGATCAAC879790.20530499865014745No Hit
TCAAGTAGGAGTGAGGCTCCCTCCTACTGAAAGAAACGCAGTTGGTGTCC800870.1868884782379245No Hit
CGCGTAGAGATATAGACGTAGACCGGGACTGAGGAATCATCAGAGTATGG797140.18601805729092005No Hit
CGCGTAGAGATATAGACGTAGACGCGTAGAATGATGGTTTCCAGCTTCAT784450.1830567592165269No Hit
CGCGTAGAGATATAGACGTAGAGGTGAGGAAGTAGAGAAACATAGATCCC766660.17890534134864236No Hit
CGCGTAGAGATATAGACGTAGAGTTCATTGAATAAGTGAACACTTTTTAA763980.17827994506500375No Hit
CGCGTAGAGATATAGACGTAGACATTACCAGCACACAACTGCCTATCCGT752520.17560567588198203No Hit
CCCGCCTTACACTGACTTCCACAATGGTTGAACTAGTTTACAGTCCCACC750210.1750666216225771No Hit
CGCGTAGAGATATAGACGTAGATACATTTCTAGGTGGTCCTACCTCCTGT748240.1746069086827383No Hit
CGCGTAGAGATATAGACGTAGAAAAGAAGATGGAAGAGCTTAGAGCCAAG734860.17148459440099043No Hit
CGCGTAGAGATATAGACGTAGAGAAGTTGGGCTGGATTTGAATCTAGACA705940.1647359151014277No Hit
TCAAGTTTCTGGCTTAGGGGCAACACGTCTGGGTTAAGAAATGGAAGAGA695230.1622366635350959No Hit
CGCGTAGAGATATAGACGTAGAAGGAAAAGAGGGAGCTGGAGCTCTGGCT660950.15423719166825603No Hit
TCAAGGTTTCTGGGTCTGAACACCTGGAAAGATAGAGTTGTCATTTACTG650760.15185928564949586No Hit
TCAAGGAGTTTAAGCTTTCTTTTCATAGAGTAGTTTGGAAACACTCTGTC649410.15154425393945403No Hit
TCAAGGATCTAGAACTAGAAATACCATTTGACCCAGCCATCCCATTACTG630570.14714781140820363No Hit
TCAAGCAATTCTCCTGCCTCAGCCTCCCAAGTAGCTGGGATTACAGGTGT629080.14680010974304636No Hit
TCAAGTAACCCTGAAAAACACTTCATTAAGTACATTTGACAAAGTTAGTT617000.14398115933022765No Hit
CGCGTAGAGATATAGACGTAGATGGAGGCCTAAGAACAGATCAGGTGCAT616700.1439111522835517No Hit
TTAAGTGTGCAAATGGTGTAATATTACATTGAATTGTTTGCACAGAGATT597960.1395380454345266No Hit
CGCGTAGAGATATAGACGTAGATGCCTCTCAGCACTACAGATAACTACCT579760.1352909512695183No Hit
TTAAGAGCTATGAGGCAAAAATATCAGGTAACCTATAAAGGAAAACCTAT576880.13461888362142907No Hit
TTAAGCGAGCCTGGGAGCAAGTGAGGGGCAGAGGTCTGATCGCAAAAAGA565310.13191894518795946No Hit
CGCGTAGAGATATAGACGTAGAGTGATTCTGAGAATGATTGATGGATCAG562080.13116520265208162No Hit
CGCGTAGAGATATAGACGTAGATGGCTCTTAGCAGTCCTAATCTTATTTG560370.13076616248602865No Hit
CGCGTAGAGATATAGACGTAGAATACAAAACAGTGTTATGAGCGGCTCAT558210.13026211174996172No Hit
CGCGTAGAGATATAGACGTAGAGCTCCTTTAGCAGTTCTTATAGTGCTGG551970.1288059651791017No Hit
TCGAGGGTAAAGCATCTTGATGTCTTTATCTGCAGGGTCTTTTCAAGCCC548960.12810356114411955No Hit
CGCGTAGAGATATAGACGTAGATATGAAATGCATCAATCTAGTGGGTAAA529240.12350176460928636No Hit
CGCGTAGAGATATAGACGTAGATGTCAAGAAATATTTGGATTCAAGTCAC520030.12135254827633435No Hit
CGCGTAGAGATATAGACGTAGAAGGGTAAAAGTGCAGACGCGGCAGGCAG518860.1210795207942981No Hit
TCAAGTGATTCTCCCACTTTGCTCCTGGAAGCAAAGTGCTGGGATTACAG518400.12097217665606162No Hit
TCAAGGCTAGGCAAATATACTCTTGCAGATTCCAGAAAAAGAGTGTTTCA518180.1209208381551659No Hit
CGCGTAGAGATATAGACGTAGAATACTGTTGAGCCTTAAAATAAAGGAAA507530.11843558799816928No Hit
TCAAGATCGGCACACAGCAGTATTCATATTTGGCTTTAAAAAGAGAGAGA502060.11715912618044423No Hit
CGCGTAGAGATATAGACGTAGATGACCCATCTAAGCCATGGTTAGATTTC498510.11633070946144536No Hit
CGCGTAGAGATATAGACGTAGATTCTAAGCTGGACATATCAGCCCTAGTG489330.11418849383316093No Hit
CGCGTAGAGATATAGACGTAGAGTGTCTTGTATGTGTGGGAAAGACAGGA480330.11208828243288209No Hit
CGCGTAGAGATATAGACGTAGATGAAATGACACTAGTTAGAGGGCTAGTC465800.10869760780554301No Hit
TTGAGATCTCATGAAGATAGGAGGTGTTTCCTATCACAGCATGTACCTGT461790.10776184694830766No Hit
TCAAGCAGAGACGGAATTCTTGCTGCCCATGGTAACAGATGCTAGCTTGA458240.10693343022930878No Hit
TCAAGTTATTGCCTCAAATACTAACTTTAAAATCTGAATATACAAGGTAC454930.10616101914765066No Hit
CGCGTAGAGATATAGACGTAGACTCCACACTTGGGTGTGTTCACCAAGGT454820.10613534989720282No Hit
CGCGTAGAGATATAGACGTAGATGTTTCCTTTTCTACCTTTGGTCTCAAT449240.10483321882902993No Hit
TTAAGAAATTGAGCATTTATTTAAAATTTCAGCTCCTAATGAATATGAGA448780.10472587469079345No Hit
CGCGTAGAGATATAGACGTAGAAAAGAAGTAGACATAAGAGACTCCATTT434510.10139587283724022No Hit
TTGAGTTTCCCACTGCAATCAGTGATACCATACTCAAAACGCAGTATGAA433260.10110417680942371No Hit
TTGAGACACTGAGAGTACACACATGCACACGCATCCACCCCCAAGCTCCA432040.1008194814862748No Hit
CGCGTAGAGATATAGACGTAGAATGACTTTGACGAGCTGAGAGAAGAAGG431630.10072380518915099No Hit
CGCGTAGAGATATAGACGTAGATTCTGGATATTAGCCCTTTGTCAGATGA430700.10050678334445551No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAG17376400.0142.4171
GCGTAGA17531750.0140.71982
CGACTGT1153450.0139.484191
CGCCTTA1036650.0137.239843
GATATAG18318900.0134.60639
CGTCTAC1213400.0133.109929
GAGATAT18707600.0131.96697
GCCTTAC1087050.0131.053024
AGAGATA18922650.0130.730386
TAGAGAT18887100.0130.645165
AGATATA19015750.0129.700038
CCGCCTT1109550.0128.025992
GCCGCCT906850.0126.848121
CTGTACG1268150.0126.705154
CGCTTAG93450.0123.040981
CCTTACA1171900.0122.410045
TGTACGT1314550.0122.125785
GTAGAGA20711200.0119.515274
CTTACAC1220500.0118.783076
TCGAGCC164100.0117.746061
Produced by FastQC (version 0.11.2)