FastQCFastQC Report
Mon 9 Apr 2018
HYKJMAFXX_n01_grads_set5_healthy_rna.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYKJMAFXX_n01_grads_set5_healthy_rna.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39364776
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGAGAGCGTAGTCGAGATACCGCGAGAGCGTAGTCGAGATACCGCGAG3582310.910029311483952No Hit
CGTGCGACGACGACGCTGTAGATGGGGCTGGGCCCGCATCATCAATGCGA2732780.6942196241634908No Hit
TCAAGCAGACCAAGGCCTACACCTACTTCAAGCCGAAATACATCTTCTAC2328880.5916152044152366No Hit
TTAAGGGCTAGTCCCCGTTCGCTCGCCACTACTAAGGGAATCTCGGTTGA1770760.44983362791140996No Hit
CGTGCGACGACGACGCTGTAGATGGTGATCCTGTGGTTCTTCCTGCTGAT1756500.4462110999945738No Hit
CGTGCGACGACGACGCTGTAGAGAGCCTCATTCGACGAGTGGGACGGAGG1672600.4248976292917302No Hit
CGTGCGACGACGACGCTGTAGATTCTTCACGTAGCGCGACAGGCCGGACG1583850.40235209264241717No Hit
TTGAGCAGGTCGTACTCGCCGACCGCATAGACCAGCGAGGTGTCCTGGAA1477000.375208536687723No Hit
TCAAGAGCCAGGGTGTCGATGAAGGTGCGATGACGCTGCAGGCAAGCAAC1467530.37280283266441044No Hit
TCGAGCGGCGGCAACACGACGCAATCGGTCGCGGGCGCGCTGCAGCAGCC1405550.3570577919711775No Hit
CGCTGACGTCGTAGCACACTCGGGTCAAGTGAAGAAGCGCATACGGTGGA1402560.3562982296660344No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1273460.3235024124105266No Hit
TTAAGCGAGCGGGCAACACGCGTCGGCTTCAGGATCAGATGGTGCCGGTG1212210.3079428166948035No Hit
CGTGCGACGACGACGCTGTAGATCATGTACCCGCGCGACCAGTATCCCGA1198770.30452859683489625No Hit
CGTGCGACGACGACGCTGTAGACCGGCAAGGGTCGTGCCGTTGCCGTATC1161000.29493372450538013No Hit
CGTGCGACGACGACGCTGTAGAACGGCATCATCACGGCAAGCGCTGCCAC1154410.2932596390234762No Hit
CGTGCGACGACGACGCTGTAGATGATCGTCGGCCCGTTGCTGATCGGCCT1142450.29022138980290396No Hit
TCGAGCACGCCCTCGTTGACCGAAGTGGCGAGGAGGTCCCACAGCAGGAA1068150.27134664757142274No Hit
TTGAGCACTTCGCGCACCCGATCGCTCTTGCTGGCGCCAACGAACAGCGC961540.2442640598285127No Hit
TCAAGAAGCCGGATTGGATTCGCGTGCGCGCCGCCACCGGCAACTCGCGC914310.23226602381784162No Hit
CGTGCGACGACGACGCTGTAGATGAGGCCAGTGCACAGGATATTTTTTTT888340.225668755234375No Hit
TCAAGCGCCTGCGGGGCTCCGCGGCGCGCGCGTCAGCAGCGCGAACACCG839280.2132058366088505No Hit
CGTGCGACGACGACGCTGTAGAGCCTTTCTCACGAAGTCTGCCAAGCAGA836960.2126164772282713No Hit
TTGAGTTCAGCGATCGTCTTGGCCAGGTTTTCCGGCTCGTTCACGTCGAG832730.21154191249557727No Hit
CGTGCGACGACGACGCTGTAGAGCGGCTGTATCGTGGTGCCTGATGGCGG820150.20834616206122955No Hit
TCAAGAACCAATTAACCAGAAATTATCTTGAAAACCTCTCCGGATCTACC806820.20495988596505665No Hit
CGTGCGACGACGACGCTGTAGACAACGCGGCGACCGCCGCGCAGGCTGAA794680.20187591058564638No Hit
CGTGCGACGACGACGCTGTAGAGCGCGGCCTGTACGCCAGCCAGATGCTG779500.19801967119030475No Hit
TCAAGCGCAATGTGCGTGCATAGTCGCTGTGATTCCAATTGCCAACGATC767490.19496872025894418No Hit
TCAAGAGCGGCCTGGCCCGCGGGCAAGGCGAGATCGCTGGCAGCCTGGTC758600.19271035608077638No Hit
CGTGCGACGACGACGCTGTAGAAGCTGGAGAAGCGCTTCGCGCAGGAGAT726460.18454569638602797No Hit
TCAAGGGAGGGGGCGCCGATTGGGCGACGGTCGATGCAAATACGATCGAC720420.18301132972279582No Hit
TCGAGCCGCGTCGAATAGCCGGGGATGTCGCCAAGCAGCAGGCCACTGGC705190.17914238861666582No Hit
TTGAGCTGCTTCAGGCGCTGGCGCTCCACGTCGTTGGTCGTCGCGGTGAT629270.15985611095564217No Hit
CGTGCGACGACGACGCTGTAGATGCTCGCCGAGCTGCAGCAGCGCAACAA623600.15841573695224379No Hit
CGTGCGACGACGACGCTGTAGAGATCACCACCAAGACGGTCGATTCATTC620040.1575113751441136No Hit
CGTGCGACGACGACGCTGTAGATGCTGGAAGAGATCGGCAGCGCCGACAA589560.14976841224753826No Hit
CGTGCGACGACGACGCTGTAGACAAGGGGACCACACAATTAATTTGATGC579580.14723315077418453No Hit
CGTGCGACGACGACGCTGTAGAGAAGGCGCAGCCTGCGCTCGACCGCGGC557890.14172314863420027No Hit
CGTGCGACGACGACGCTGTAGAGAGCTGGAACTGGTGGCCGCGCTGCACG545900.13867727838715505No Hit
TTAAGAATGTCATCGTAGATGACCTTCATTTCCCGTGTGGGCCATTCTGA537700.13659419781786641No Hit
CGTGCGACGACGACGCTGTAGAAGGGACCGTATGAGGGCCGGGCTACTGG536400.13626395333736943No Hit
TCAAGCTCCTTCAGCGCTGCCTGGGTGAACTTCTCGCCGAGTGCCTTGGA533090.13542310008318098No Hit
CGATACAGCTACTACTACTCTATGAAAAGAAAGGTTAAACTCTGTGAGTT521080.13237214915182038No Hit
CGTGCGACGACGACGCTGTAGATTCTGGATATTAGACCTTTGTTGGATGT519940.13208255014584613No Hit
CGTGCGACGACGACGCTGTAGATGGCTCCAACCCTGGGGAGTGTACTTTC519850.1320596870664271No Hit
CGTGCGACGACGACGCTGTAGACCGTCTTGCCGGATGCCCGCATCGTCAT518900.13181835456144853No Hit
CGTGCGACGACGACGCTGTAGAGTTGTATCATTATTTGTCATAATTTCAC516970.1313280685250184No Hit
CGTGCGACGACGACGCTGTAGAGCGCATCCTGGACGTGCTGCTGCCCGGC514050.13058628861497903No Hit
TTGAGTAATCTGCAAGGACTCTTCTGGGTTTGCAAACCAGACTCCCCCCT507580.12894268723896712No Hit
CGTGCGACGACGACGCTGTAGAAGTAGCACTCGCCCTGGATTGCGCCGTA503990.12803070440436395No Hit
TTGAGCTGCTCGGCCATGTCCTCCATGCCGGGCTGGTTGCCAAAGGGCGT498540.12664621792843428No Hit
CGTGCGACGACGACGCTGTAGATGACGGCGGCATCGGGGTCGGCGCGGTG491640.12489338183964262No Hit
CGTGCGACGACGACGCTGTAGAGCGCCTGCCCGACGGCCTGGTGCTCACC489250.12428624006395972No Hit
CGTGCGACGACGACGCTGTAGACGACGCTGTAGAAGACGCGGTGGCGCAG488850.12418462637765294No Hit
CGTGCGACGACGACGCTGTAGAAAGCGCAGGTCGACCATCCGCTGCCGCC478760.12162142114056486No Hit
TTGAGCAGGTAGGCGGCTCCTAGCTCGGGGTAGTTCTTCGTCCATCCGTC477810.1213800886355863No Hit
TTAAGGAGGCTGGTATCAAATACAAAGTTGGTAAATTCCCATTTATTGCT477540.12131149939732923No Hit
TCGAGCTTCACCTACGCGCAGGCGACGTGGACGCAGGGGCTCGCCGACTG474390.12051129161766347No Hit
CGTGCGACGACGACGCTGTAGATCCTGTGGGAGGAGTACATCGGCGCCGA473210.12021153124305853No Hit
TTGAGGCTGACATGGATCGTGTCCTGCGGCAGGCCCGCATGGCCGTAGAT473070.12017596645285114No Hit
CGTGCGACGACGACGCTGTAGAGGGCAAGCCGCTGATCGTGCTGATCAAC472110.11993209360571491No Hit
CGTGCGACGACGACGCTGTAGATGTCGACCTCCGTCAATGAGCCGACCTT469730.11932749217218967No Hit
CGTGCGACGACGACGCTGTAGATCGGCCAGAAGACGCTGCTGCGCCTCTC469540.11927922567119398No Hit
CGTGCGACGACGACGCTGTAGAAGCACCGTATTCTAAAGCAAGCCCAGCC464690.11804715972472445No Hit
TCGAGGGTGAAGGCAACGACTATGTCGGCAAGGGCCTGTCGGGCGGCAAG464320.1179531670648907No Hit
TCAAGATGGATCAAGGACTTAAATCTAAGACCTGAACCATAAAAATTCTA460340.1169421108861384No Hit
TTGAGCACCTTGCTGGTCATCTCACCGATGACCTTGGCGCGCGGATCGAA458890.11657376127327639No Hit
TCGAGATCCCGGATGCGCGCTAAGCTCGCTCCGGGATGACGCGCTTTCGC456140.1158751671799174No Hit
CGTGCGACGACGACGCTGTAGAAGGACGGCCTGCTCTACCGCAACAAGGT452700.11500128947767924No Hit
TCGAGAACGACTGGCCCTGCGGCGTCGTCAGTTCGGGGTGGTCCCACACG451360.11466088362855158No Hit
TTGAGTGTGATCGCGCCCTGTGTGAAGTGGCCCACCTGAGCCACCGGGTG444620.1129486930142826No Hit
TTGAGCTTGGACGTGCCATGGCCTTCGATCTTGGCGCCCAGCAGCGACAT444250.11285470035444886No Hit
TCGAGGCGCCGAGCACGGCGACGAAGATCTGCGCCGTCCTCCGCTCGCCG441350.11211800112872483No Hit
CGTGCGACGACGACGCTGTAGACCCCGCCCTTGGATCTGGGAAATTGGAT435360.11059633617628103No Hit
CGTGCGACGACGACGCTGTAGAACGTCAGCAACCCCGACATCAAGCAGCT424730.10789595246267882No Hit
TTGAGTTGCGGCACAGCATCGGGAGCAGGGGTCTCGATGAGATCGACCTG424520.10784260527736778No Hit
TCGAGTTCGACGGTCCGGCGGGTGCGGCGCCGGGCGTCGTGCAGCGTGAC418410.10629045621903196No Hit
TCGAGCTGTATCATGCCGTCGTGACCGGCGTCGCCGACGCAACGAAAACG413630.10507617266766614No Hit
CGTGCGACGACGACGCTGTAGAGATTGGCCGGGTGATCACCGATTTTGAT412920.10489580837447163No Hit
TCAAGCCAGTTGGTGGCGGTCAGGCGCTTCCACATGGAAACCTTTCAGTT411400.10450967636650593No Hit
CGTGCGACGACGACGCTGTAGATGTGAAATTTGATTAATTATACATATTG407470.103511321898542No Hit
TCAAGGTGCTGGCCCATGCCAACGAACTCAGGCGTGGGCGGCTGATGGCC404350.10271873514534924No Hit
TTGAGCAATATTTTGACCGAATCAACCAGGTCTATGTTGTTCTGAAAGTG403000.10237578895406389No Hit
CGTGCGACGACGACGCTGTAGAACATCGACCGCGGGTCCGGCGTCGCCTG399990.1016111459646055No Hit
CGTGCGACGACGACGCTGTAGAAACCCGCGTACTGGTCGGAGGACCGGCT397160.10089222913398517No Hit
TCAAGATCACCGGCGTGCAGGAGCTCGGCGGCGCCGACTACAGCTTCACC394190.10013774751315745No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCGAC8308800.0142.47322
CGTGCGA7939050.0141.51831
TGCGACG8489900.0139.494373
ACGACGA8684350.0136.367317
CGATACA3083100.0134.130511
GCGACGA8887500.0132.942734
GCGACTA1102600.0132.006042
CACGCAC1164250.0125.155849
TACACGC1173000.0124.479887
CGCGACT1145000.0123.896211
ACACGCA1184800.0122.95458
CTACACG1213350.0120.494886
TGACGTC1116150.0118.9640664
TTAAGCG170300.0118.9587551
CTGACGT1135050.0116.767493
ATACAGC3778450.0114.907493
ACTACAC1272600.0114.73215
GTCGTAG1179500.0113.27638
TAAGCGA175400.0112.3936842
CGTCGTA1189550.0112.289017
Produced by FastQC (version 0.11.2)