FastQCFastQC Report
Mon 9 Apr 2018
HYKJMAFXX_n01_grads_set5_healthy_dna.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYKJMAFXX_n01_grads_set5_healthy_dna.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42852829
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGCCTTACACTGACTTCCACAATGGTTGAACTAGTTTACAGTCCCACC4635991.081839894397637No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3076480.717917596525541No Hit
CGACTGTACGTCTACATCGTCAGTGGCCATCAGAGAAATGCAAATCAAAA2432130.5675541281066881No Hit
CGACTGTACGTCTACATCGTCACTGGCCATCAGAGAAATGCAAATCAAAA2285140.5332530088036895No Hit
TCAAGTTTTTGCATTTCCTTTTCATGTTCTACTTCGTGATTTCTTTTTTA2103900.4909594183385185No Hit
TCAAGCCTCTCCCTGAGCCTGGGGACACAGGGACAGCATCAGAAATGGAT1765150.4119097948002453No Hit
CGCGTAGAGATATAGACGTAGAGAAGAGCAAACGCCTCCTGGCAGGCGGA1550990.3619340977464988No Hit
TCAAGGAGTTCACTGTCTAACAGAAGAGACAGAGAAGACAACTAAGCAGC1441640.33641652923311083No Hit
CGCGTAGAGATATAGACGTAGAGTGTGTTCAGGTGTGTGTGTGATGGGGC1349060.3148123546289091No Hit
TCAAGTAGGAGTGAGGCTCCCTCCTACTGAAAGAAACGCAGTTGGTGTCC1251100.2919527203209851No Hit
TCGAGAACTACGTGAAGAATGCAGAAGCCTCAGGAGCCGATGCGATCAAC1188890.2774355924086132No Hit
CGCGTAGAGATATAGACGTAGATACAGTGTTTATGTCAAGTGCAGCTATA1155180.26956913393045767No Hit
TTGAGTGTGTTTTTGAAGGAGCATGTTTCAGTTCTTTGTTTTCTGTTTGC1132090.26418092490463113No Hit
CGCGTAGAGATATAGACGTAGATTTGGTCTTTTCACATAGTCCCATATTT1072510.2502775254347852No Hit
CGCGTAGAGATATAGACGTAGAGAATGTAAAGCCACCACACCACTAAGTC1060520.24747957713596924No Hit
TTGAGACCAGGAGTTCCAGGCTGCAGTGAACTGTGATCACACCACTGAAC985710.230022153263207No Hit
CGCGTAGAGATATAGACGTAGACACACTGCGCTAGATACAGATACAGAGC974740.22746222892308932No Hit
CGCGTAGAGATATAGACGTAGAACATTCCCTTTGGTAGAGCAGGTTTGAA868730.20272407219602703No Hit
TCAAGGATCTAGAACTAGAAATACCATTTGACCCAGCCATCCCATTACTG830770.19386584722329536No Hit
TCAAGGTTTCTGGGTCTGAACACCTGGAAAGATAGAGTTGTCATTTACTG826210.19280174011382073No Hit
TCAAGTTTCTGGCTTAGGGGCAACACGTCTGGGTTAAGAAATGGAAGAGA820800.1915392797054309No Hit
TCAAGGAGTTTAAGCTTTCTTTTCATAGAGTAGTTTGGAAACACTCTGTC804950.1878405740727176No Hit
TCAAGCAGAGACGGAATTCTTGCTGCCCATGGTAACAGATGCTAGCTTGA797250.18604372654136792No Hit
CGCGTAGAGATATAGACGTAGACAGGCAGTCCTGGGCACAGCCTCCCATC795010.1855210072595207No Hit
TCAAGCAATTCTCCTGCCTCAGCCTCCCAAGTAGCTGGGATTACAGGTGT792830.18501228938700873No Hit
TTAAGCGAGCCTGGGAGCAAGTGAGGGGCAGAGGTCTGATCGCAAAAAGA787790.18383617100285257No Hit
TTAAGTGTGCAAATGGTGTAATATTACATTGAATTGTTTGCACAGAGATT782790.18266938689158657No Hit
TTAAGAGCTATGAGGCAAAAATATCAGGTAACCTATAAAGGAAAACCTAT772080.18017013532525472No Hit
CGCGTAGAGATATAGACGTAGACCGGGACTGAGGAATCATCAGAGTATGG739980.17267938133092683No Hit
TCAAGTAACCCTGAAAAACACTTCATTAAGTACATTTGACAAAGTTAGTT720210.16806591695498097No Hit
TCGAGGGTAAAGCATCTTGATGTCTTTATCTGCAGGGTCTTTTCAAGCCC690260.16107688012849747No Hit
CGCGTAGAGATATAGACGTAGACGCGTAGAATGATGGTTTCCAGCTTCAT668040.15589169153803123No Hit
CGCGTAGAGATATAGACGTAGAAAAGAAGATGGAAGAGCTTAGAGCCAAG666490.15552998846353877No Hit
CGCGTAGAGATATAGACGTAGAGGTGAGGAAGTAGAGAAACATAGATCCC655090.15286972068985225No Hit
CGCGTAGAGATATAGACGTAGACATTACCAGCACACAACTGCCTATCCGT647290.15104953747627725No Hit
TCAAGGCTAGGCAAATATACTCTTGCAGATTCCAGAAAAAGAGTGTTTCA632840.14767753139471843No Hit
TCAAGTGATTCTCCCACTTTGCTCCTGGAAGCAAAGTGCTGGGATTACAG619530.14457155209052827No Hit
CGCGTAGAGATATAGACGTAGAGAAGTTGGGCTGGATTTGAATCTAGACA604390.14103852980161471No Hit
CGCGTAGAGATATAGACGTAGAGTTCATTGAATAAGTGAACACTTTTTAA604330.14102452839227952No Hit
TTAAGAAATTGAGCATTTATTTAAAATTTCAGCTCCTAATGAATATGAGA601270.14031045651618473No Hit
TCAAGATCGGCACACAGCAGTATTCATATTTGGCTTTAAAAAGAGAGAGA586870.13695011827573858No Hit
CGCGTAGAGATATAGACGTAGATACATTTCTAGGTGGTCCTACCTCCTGT579150.13514860360794384No Hit
TCAAGTTATTGCCTCAAATACTAACTTTAAAATCTGAATATACAAGGTAC577720.13481490335212173No Hit
TTAAGTCAAATGTCACAGAACAAACTTACCAGGGATGTATCTATCTAATA569880.1329853858656566No Hit
TTAAGATCGAATTTTTTACCAAATTACAAGTATGCAATATAGTATGATTA553260.12910699547980833No Hit
TCAAGCTAATAGCTGTTAATATCTTAGGATTACATCTCTTTAGAACAGAT543570.12684576787217478No Hit
CGCGTAGAGATATAGACGTAGATGGAGGCCTAAGAACAGATCAGGTGCAT540850.12621103731564606No Hit
TTGAGATCTCATGAAGATAGGAGGTGTTTCCTATCACAGCATGTACCTGT536970.12530561284530364No Hit
TCAAGGATCCATGCTGATGGAAGGTTGCCATCTTCTGCACATGGCTTCTA534890.12482023065501698No Hit
TTAAGAGTGTTGATGTATAGAATGCCACAAACCATAACAAAGAATGTGGG516040.12042145455554405No Hit
CGCGTAGAGATATAGACGTAGATGGCTCTTAGCAGTCCTAATCTTATTTG506810.11826757108614695No Hit
TCAAGATTTCTCAACGAAAAACCATTCACAGGTGTTAGATGGGATGCATG504050.11762350625672811No Hit
TCAAGGGCAGTACCAGGTACAAGAGGCCTTCCTACTGCAGTTCCCAAGTG491110.11460386897677163No Hit
TCAAGTAGCTGTGACTGAAGGAACACACCACCATACCCAGCTATAATAGT481720.11241264841581405No Hit
TTGAGTTTCCCACTGCAATCAGTGATACCATACTCAAAACGCAGTATGAA479880.11198327186286813No Hit
CGCGTAGAGATATAGACGTAGATGTCAAGAAATATTTGGATTCAAGTCAC475440.11094716757206391No Hit
CGCGTAGAGATATAGACGTAGAATACAAAACAGTGTTATGAGCGGCTCAT462360.107894860336992No Hit
TCAAGGGCATTGCGGCACAAAGCTAAATTTGGCTGCTGCATTTCCATGGG461780.10775951338008513No Hit
CGTATCATCGACGTAGTCTGTCGTATCATCGACGTAGTCTGTCGTATCAT460330.10742114598781799No Hit
CGCGTAGAGATATAGACGTAGATGCCTCTCAGCACTACAGATAACTACCT458210.10692642952464118No Hit
TTGAGTGTATATTCTGACTTCAAAACTTCTAACTATGTAGGCAAACAGTG455130.10620769051210133No Hit
CGTAGTGCGTAGTACGTAGAGACGTAGTGCGTAGTACGTAGAGACGTAGT454040.10595333157584531No Hit
TCAAGTCTCCCAGAGCATTCTGGGAGCAGAGTTTTTAAGGACAACTTGGT449060.10479121460102436No Hit
CGCGTAGAGATATAGACGTAGATGAAATGACACTAGTTAGAGGGCTAGTC447300.10438050659385871No Hit
CGCGTAGAGATATAGACGTAGAAGGGTAAAAGTGCAGACGCGGCAGGCAG445400.10393712863157763No Hit
TCAAGGATATTGACACCAATAACTAAATTCTAGTATATTAAGCTTGATAT444700.10377377885600039No Hit
CGCGTAGAGATATAGACGTAGATATGAAATGCATCAATCTAGTGGGTAAA444150.10364543260376112No Hit
TCAAGATGGATTAAAGACTTAAATGTAAGACCTAAAACCATTAAAAATCC436600.10188358859574943No Hit
CGTATCATCGACGTAGTCTGTCCGTATCATCGACGTAGTCTGTCCGTATC435580.10164556463705114No Hit
TCAAGAACATGAAGTTATTCTGATGCTGCTAAATGTAAAACAGTAGGTCA435560.10164089750060608No Hit
CGCGTAGAGATATAGACGTAGAGTGATTCTGAGAATGATTGATGGATCAG432910.1010225019216351No Hit
TTAAGATTTTGTCTTTTTTGGAGGGTCATGGAATAATTATTAACAATAGC430420.1004414434342246No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAG13433000.0141.861941
GCGTAGA14763900.0139.766242
CGACTGT1295150.0139.548661
CGCCTTA1043300.0136.615193
CGTCTAC1379150.0133.788259
GATATAG15802350.0132.878789
GCCTTAC1087450.0131.287164
AGAGATA16053350.0130.77956
GGCGTAG1196900.0129.703521
GAGATAT16188500.0129.701317
GCCGCCT1087050.0129.510221
TAGAGAT16252100.0129.012125
AGATATA16346300.0128.471258
CTGTACG1451400.0127.400894
CCGCCTT1135300.0125.488982
TGTACGT1485650.0124.3426365
CCTTACA1179100.0121.094585
TCGAGCC183050.0119.991381
CTTACAC1193550.0119.67656
ACTGTAC1568750.0117.531053
Produced by FastQC (version 0.11.2)