FastQCFastQC Report
Tue 28 Jan 2020
HYKFCBGXC_n02_Salt_NSF_Plate_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYKFCBGXC_n02_Salt_NSF_Plate_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences341327880
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44586601.3062689165619872No Hit
GTGTTATACCGGTGGCTTCAGGGGGTATTCATGTTTGGCATATGCCAGCT9353080.2740203935289435No Hit
GTCATATATAGCCTCGTTTAATTGTTCATCTCTGATTCGATGATGTCTCC5605050.16421307277917058No Hit
TCCTTGGGGTAATGCACCTGGTGCAGCAGCTAATCGGGTGGCTTTAGAAG4529350.1326979208378759No Hit
GTACTGGACCATGTGGAAGCTGCCCATGTTCGGGTGCACCGACGCCACCC3552730.1040855496480393No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA2782850.021.1028771
GGTATCA841850.021.0845931
GGACCAT2670000.019.5652336
GTTATAC2907300.018.719023
TAGATCT1283700.018.60678144
TGGACCA3028950.018.2669935
GGGGGTA2883900.018.25727321
GTAGATC1332800.018.2529443
TCAGGGG2906950.018.0170518
GGGGTAT2885800.018.00350622
ATACCGG3078900.017.9821536
GTACTGG2340550.017.9384751
GGTATTC2918600.017.67605624
GGGTATT2956000.017.59451723
TGGCATA2826950.017.52300536
TACCGGT3140600.017.4586817
GGCTTCA2999350.017.45756514
TGTTATA3221950.017.2849332
CAGGGGG3033750.017.26760719
AGGGGGT3046450.017.21876520
Produced by FastQC (version 0.11.5)