FastQCFastQC Report
Tue 28 Jan 2020
HYKFCBGXC_n01_Salt_NSF_Plate_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYKFCBGXC_n01_Salt_NSF_Plate_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences341327880
Sequences flagged as poor quality0
Sequence length20
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGG66337181.9435031208115787No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACACT3688650.013.8790342
TCCTTAC4576450.013.854024
AATCCTT4573450.013.8511112
CCTTACG4576800.013.8443965
TCAACCG3471050.013.8374821
CCTCTCT2404100.013.8350954
AGAGATC6390000.013.8313223
ACACCTT3084100.013.8179262
TACACCT3086700.013.8178171
TTCCACC2343350.013.8178112
CCTCAGT3325000.013.8143946
CCACTCT3231550.013.8079725
GGTCCAA4515850.013.8075563
TCGACCA3392800.013.804142
AACCGAT3486750.013.8030053
CCGTGAC4807700.013.7960614
CTTCGTC4045700.013.7943522
CGCATCC3321750.013.7931415
GAATCCT4593950.013.7918861
CGGAATA4331100.013.790461
Produced by FastQC (version 0.11.5)