FastQCFastQC Report
Wed 7 Mar 2018
HYKCJAFXX_n01_ah9048_5-95_spike-in_input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYKCJAFXX_n01_ah9048_5-95_spike-in_input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5188042
Sequences flagged as poor quality0
Sequence length100
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC57610.11104381961441329TruSeq Adapter, Index 3 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC21150.022.44258548-49
AAGGGGG21300.019.30963372-73
CGTATGC25800.019.3088544-45
TATGCCG25800.018.67075546-47
ACTTAGG25950.018.56318932-33
GCATCTC27900.016.76106838-39
CTCGTAT27350.016.66812542-43
AGGCATC28300.016.10898236-37
TTAGGCA30350.015.94971734-35
CCGTCTT30200.015.63988650-51
GCCGTCT22100.014.99197148-49
ATCTCGT29200.014.88789640-41
AGGGGGG22550.014.59140572-73
GAGCACA47650.014.2021179
CCAGTCA35050.014.07831326-27
CTTAGGC23800.014.01998132-33
AGTCACT37050.013.12808828-29
GTATGCC26400.013.08439544-45
AGAGCAC52100.013.0794058
CGTCTGA38050.012.65955616-17
Produced by FastQC (version 0.11.2)