FastQCFastQC Report
Wed 7 Mar 2018
HYKCJAFXX_n01_ah9048_5-95_spike-in_chip.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYKCJAFXX_n01_ah9048_5-95_spike-in_chip.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5008790
Sequences flagged as poor quality0
Sequence length100
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC52370.10455619021759748TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC22900.022.16442348-49
CGTATGC25450.020.40487944-45
TATGCCG25350.020.29947746-47
AAGGGGG20300.019.9133572-73
CGATCTC21600.019.79977238-39
CACGATC23350.018.91957336-37
CTCGTAT28250.016.46975342-43
CCGTCTT30900.015.97026350-51
ATCTCGT30150.014.65239940-41
ATCACGA34250.014.47609534-35
CCAGTCA38500.013.85406926-27
AGGGGGG17650.013.8484372-73
AGTCACA39900.013.19129228-29
TCACATC43900.012.04311630-31
CGTCTGA43550.011.92384416-17
CTCCAGT45600.011.80002224-25
CACGTCT45350.011.39875914-15
GCCGTCT22350.011.24979148-49
ACATCAC46350.011.1532232-33
GTATGCC24650.010.96252144-45
Produced by FastQC (version 0.11.2)