FastQCFastQC Report
Wed 7 Mar 2018
HYKCJAFXX_n01_ah9048_30-70_spike-in_input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYKCJAFXX_n01_ah9048_30-70_spike-in_input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5695261
Sequences flagged as poor quality0
Sequence length100
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC24750.022.59624350-51
TATGCCG28150.020.36734848-49
CGTATGC30500.019.02901646-47
AAGGGGG27450.017.55367574-75
CTCGTAT31700.017.19703944-45
CCGTCTT33850.016.4523552-53
ATCTCGT31650.016.40768642-43
AGTCACA36350.016.03086528-29
CCAGTCA36150.015.92449126-27
CGTCTGA38700.014.75390216-17
CACGTCT40600.013.83189114-15
ACATTCC43700.013.3887232-33
CACACGT42550.013.19793912-13
CTCCAGT46450.012.39333324-25
GCCGTCT24500.011.79705250-51
TGCTTGA49150.011.71375560-61
TCTGAAC51600.011.61186818-19
AGCACAC50400.011.28216910-11
GAGCACA52100.011.2748329
CTTCTGC50650.011.18077956-57
Produced by FastQC (version 0.11.2)