FastQCFastQC Report
Wed 7 Mar 2018
HYKCJAFXX_n01_ah9048_30-70_spike-in_chip.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYKCJAFXX_n01_ah9048_30-70_spike-in_chip.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6135527
Sequences flagged as poor quality0
Sequence length100
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT112150.18278788439852028TruSeq Adapter, Index 25 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC32300.026.91780750-51
CGTATGC37200.023.75051146-47
TATGCCG37650.023.5291548-49
CCGTCTT39300.022.54247752-53
CTCGTAT39950.022.0568944-45
AAGGGGG32750.021.10052774-75
ATCTCGT44350.019.76304442-43
GCCGTCT30750.019.56299250-51
AGGGGGG27400.018.87249874-75
AGTCACA48800.018.24875628-29
CCAGTCA52650.016.95902426-27
GTATGCC35800.016.93420246-47
TATCTCG37000.016.70279540-41
TCACACT54600.016.56858830-31
ATGCCGT38050.016.24167848-49
GTCACAC39900.015.3113828-29
ACACTGA59100.015.14835732-33
GAGCACA82350.014.7233069
CTCCAGT61250.014.69298824-25
TGCTTGA61700.014.43437760-61
Produced by FastQC (version 0.11.2)