FastQCFastQC Report
Wed 7 Mar 2018
HYKCJAFXX_n01_ah9048_25-75_spike-in_input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYKCJAFXX_n01_ah9048_25-75_spike-in_input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6013305
Sequences flagged as poor quality0
Sequence length100
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT88330.14689093601605108TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC25800.022.2225250-51
ACGAGTG29900.019.48947332-33
TATGCCG31000.018.57036248-49
CGTATGC31850.018.5174146-47
TCACGAG33050.017.91616630-31
AGTCACG33150.017.79109228-29
GCCGTCT27050.017.46031250-51
CTCGTAT33700.017.08309744-45
CGAGTGG28500.016.98407732-33
CACGAGT28500.016.98393630-31
GAGTGGA36800.016.09087234-35
AAGGGGG28200.016.00327974-75
ATCTCGT35400.015.93083142-43
CCGTCTT36300.015.92457952-53
TATCTCG29200.015.85288840-41
AGGGGGG27400.015.35533674-75
GTATGCC30650.015.33258946-47
GTCACGA32050.015.17590828-29
ATGCCGT31900.015.17373648-49
GAGCACA67950.014.1778519
Produced by FastQC (version 0.11.2)