FastQCFastQC Report
Wed 7 Mar 2018
HYKCJAFXX_n01_ah9048_25-75_spike-in_chip.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYKCJAFXX_n01_ah9048_25-75_spike-in_chip.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6267178
Sequences flagged as poor quality0
Sequence length100
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTA150520.24017189235729383TruSeq Adapter, Index 22 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT91250.14559982180177425TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC41150.032.20757350-51
CGTACGT44900.029.72673634-35
TATGCCG46200.029.09326248-49
CGTATGC47650.028.01035146-47
CCGTCTT48300.027.34324352-53
AAGGGGG40950.026.40193774-75
CTCGTAT49550.025.98834844-45
ACCGTAC52900.025.14145532-33
ATCTCGT51000.024.28226342-43
CGTAATC56300.022.20532638-39
AGTCACC63050.021.65278628-29
CCAGTCA65100.021.11523426-27
TCACCGT67000.020.06092330-31
TACGTAA70000.019.26930436-37
CCGTACG42250.019.02149634-35
TAATCTC67250.018.86905940-41
GCCGTCT41700.018.70953252-53
TGCTTGA71900.018.33595360-61
CTCCAGT75250.018.26713224-25
GTATGCC44800.018.04341548-49
Produced by FastQC (version 0.11.2)