FastQCFastQC Report
Wed 7 Mar 2018
HYKCJAFXX_n01_ah9048_20-80_spike-in_input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYKCJAFXX_n01_ah9048_20-80_spike-in_input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4863623
Sequences flagged as poor quality0
Sequence length100
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG21350.023.33231228-29
TGCCGTC19200.021.53972650-51
CCAGTCA26600.019.16873426-27
TATGCCG23500.018.69812248-49
CGTATGC22750.018.69482446-47
CACGTCT27950.018.49517414-15
CACACGT28450.018.17031312-13
GTTTCGG28700.017.60276234-35
CGTCTGA29200.017.38145816-17
AAGGGGG22450.017.27513174-75
CTCGTAT24850.017.02051244-45
TCACGTT31550.016.08721530-31
CGGAATC29100.015.98792238-39
CCGTCTT26350.015.78472452-53
ATCTCGT26500.015.60606142-43
AGATCGG32700.015.3844611
CTCCAGT34000.015.20404324-25
AGCACAC34750.014.943910-11
GATCGGA36250.014.3966431
ACGTTTC35050.014.21262632-33
Produced by FastQC (version 0.11.2)