FastQCFastQC Report
Wed 7 Mar 2018
HYKCJAFXX_n01_ah9048_20-80_spike-in_chip.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYKCJAFXX_n01_ah9048_20-80_spike-in_chip.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6948112
Sequences flagged as poor quality0
Sequence length100
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT82620.1189100002993619TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC33100.024.20875750-51
TATGCCG36000.022.323448-49
ACGTGGC36750.021.9319232-33
GGCCTTA37050.021.37432936-37
CGTATGC38350.020.71015546-47
TCACGTG40250.020.14090730-31
AGTCACG41300.019.85628928-29
CTCGTAT40850.019.04100444-45
CCGTCTT42150.018.95577452-53
ATCTCGT39550.018.71621342-43
AAGGGGG35700.017.90689374-75
GTGGCCT47050.017.28087434-35
AGGGGGG26550.016.28819374-75
CCAGTCA54300.015.31884826-27
GCCGTCT31550.015.2686550-51
CGTGGCC34150.014.51882932-33
CACGTGG36850.013.64589730-31
ATGCCGT38300.013.62043448-49
GTATGCC37300.013.41846546-47
CCTTATC59950.012.89621438-39
Produced by FastQC (version 0.11.2)