FastQCFastQC Report
Wed 7 Mar 2018
HYKCJAFXX_n01_ah9048_15-85_spike-in_input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYKCJAFXX_n01_ah9048_15-85_spike-in_input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5756276
Sequences flagged as poor quality0
Sequence length100
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC21000.017.79154248-49
AGTCACG22900.016.10932528-29
TCACGGC22750.015.80247530-31
CGTATGC23900.015.436233544-45
GCCGTCT20600.015.1712148-49
CACGGCT20700.014.870172530-31
ACGGCTA24200.014.856276532-33
GTCACGG21650.014.54320328-29
TATGCCG27100.014.04650746-47
GGCTACA25600.013.952579534-35
CTCGTAT26900.013.88942942-43
CGGCTAC22800.013.60419932-33
CCGTCTT28500.013.19218150-51
CATCTCG22350.013.03752138-39
GTATGCC24400.012.80859344-45
AAGGGGG22800.012.78208972-73
ATCTCGT29000.012.72158540-41
GAGCACA50300.012.6133829
AGGGGGG22050.012.47074272-73
AGAGCAC51350.012.3554658
Produced by FastQC (version 0.11.2)