FastQCFastQC Report
Wed 7 Mar 2018
HYKCJAFXX_n01_ah9048_15-85_spike-in_chip.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYKCJAFXX_n01_ah9048_15-85_spike-in_chip.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6535664
Sequences flagged as poor quality0
Sequence length100
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC24200.019.61343848-49
TATGCCG27700.017.04985446-47
CGTATGC27750.016.6805144-45
AAGGGGG27350.015.8122272-73
CTCGTAT32350.014.01820642-43
CCGTCTT34550.013.1943550-51
ATCTCGT37300.012.47270940-41
ACTAGCT41050.012.24926432-33
GCTTATC41450.012.074738536-37
TCACTAG42300.011.88701630-31
CCAGTCA43350.011.70749726-27
AGGGGGG19350.011.66176174-75
TAGCTTA45300.011.20404734-35
AGTCACT46500.011.11644328-29
GCCGTCT23400.010.34321850-51
CTCCAGT51150.010.19780624-25
CGTCTGA53700.09.6696516-17
CACGTCT52600.09.60377914-15
GTATGCC28000.09.14687846-47
CACACGT57550.08.85935712-13
Produced by FastQC (version 0.11.2)