FastQCFastQC Report
Wed 7 Mar 2018
HYKCJAFXX_n01_ah9048_10-90_spike-in_input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYKCJAFXX_n01_ah9048_10-90_spike-in_input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5639912
Sequences flagged as poor quality0
Sequence length100
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC95050.16853099835600271TruSeq Adapter, Index 9 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG64690.11470037121146572TruSeq Adapter, Index 9 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC32400.026.907248-49
TATGCCG34450.025.5146-47
CGTATGC37400.023.6868644-45
AAGGGGG31200.021.77036372-73
AGTCACG40550.021.67172228-29
CCGTCTT40700.021.53555150-51
CTCGTAT38900.021.50550742-43
ATCTCGT39300.020.38971140-41
AGATCTC42500.018.6888638-39
TCACGAT48550.018.48770330-31
CCAGTCA50000.017.90480426-27
ACGATCA49350.017.71242732-33
GCCGTCT30000.017.62385648-49
GATCAGA49550.017.26194434-35
TGCTTGA55700.016.2857758-59
TCAGATC50400.016.0856236-37
GATCTCG31300.015.84138638-39
GTATGCC34850.015.30596844-45
CTCCAGT59700.014.99564824-25
CGTCTGA58450.014.95440716-17
Produced by FastQC (version 0.11.2)