FastQCFastQC Report
Wed 7 Mar 2018
HYKCJAFXX_n01_ah9048_10-90_spike-in_chip.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYKCJAFXX_n01_ah9048_10-90_spike-in_chip.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6053717
Sequences flagged as poor quality0
Sequence length100
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC23450.020.74199948-49
TATGCCG26650.018.60370346-47
AAGGGGG25100.017.88572372-73
CGTATGC30100.016.78398544-45
CCGTCTT31900.014.58500550-51
CTCGTAT32150.014.178884542-43
AGGGGGG19400.013.93294772-73
ATCTCGT33150.013.82181940-41
AGTCACA41700.013.24127528-29
TCACACT43750.012.88935430-31
CCAGTCA43950.012.61695926-27
ACACTTG45550.012.27699132-33
GCCGTCT22400.011.7487948-49
CGTCTGA50300.010.97754116-17
CTCCAGT53300.010.53591524-25
CACGTCT52600.010.40810714-15
GAATCTC46350.010.18976238-39
ATGCCGT28400.010.09382246-47
GTATGCC29550.010.01927244-45
CACACGT54900.09.80083112-13
Produced by FastQC (version 0.11.2)