FastQCFastQC Report
Wed 7 Mar 2018
HYKCJAFXX_n01_ah119_5-95_spike-in_input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYKCJAFXX_n01_ah119_5-95_spike-in_input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5553681
Sequences flagged as poor quality0
Sequence length100
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG459720.8277753079444067TruSeq Adapter, Index 4 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC124580.22431968994978285TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG82050.065.656111
GATCGGA94450.056.9291272
AGAGCAC99350.054.0208939
TCGGAAG102850.052.3248184
ATCGGAA109450.049.5122033
CGGAAGA111300.048.310045
GAAGAGC120350.044.8676767
GGAAGAG129350.041.8597376
AAGAGCA130300.041.3697178
TGCCGTC83350.040.14523748-49
ACTGACC83800.039.53727332-33
CGTATGC87650.038.3376544-45
CACACGT89950.038.0608312-13
CGTCTGA89950.038.06065816-17
TATGCCG88700.037.9880846-47
CACGTCT91550.037.34431514-15
CCGTCTT90300.036.7701850-51
CTCGTAT84600.036.49777642-43
CCAGTCA92900.036.47249626-27
AAGGGGG75700.036.32451272-73
Produced by FastQC (version 0.11.2)