FastQCFastQC Report
Wed 7 Mar 2018
HYKCJAFXX_n01_ah119_5-95_spike-in_chip.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYKCJAFXX_n01_ah119_5-95_spike-in_chip.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6416456
Sequences flagged as poor quality0
Sequence length100
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATG378060.5892037598325306TruSeq Adapter, Index 2 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC184820.2880406255415762TruSeq Adapter, Index 2 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG81990.12778081857025125No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC79350.037.43095848-49
CGTATGC86850.035.01052544-45
TATGCCG86850.035.0099846-47
AAGGGGG72500.034.45936672-73
CCGTCTT86650.033.65405350-51
CTCGTAT89850.032.08945542-43
ATCTCGT93800.029.760940-41
CGATGTA105100.029.46785534-35
GTATCTC96600.028.9964738-39
ACCGATG113350.027.75734732-33
TGCTTGA102550.027.47544558-59
CTTCTGC106250.027.09359754-55
ATGTATC113000.025.6613436-37
GCCGTCT77450.025.33373350-51
ATGCCGT81800.024.90557748-49
TCACCGA128400.024.72317130-31
GTCTTCT118450.024.3223152-53
AGGGGGG68750.024.23954674-75
GTATGCC85650.023.97791746-47
AGTCACC132150.023.86167328-29
Produced by FastQC (version 0.11.2)