FastQCFastQC Report
Wed 7 Mar 2018
HYKCJAFXX_n01_ah119_30-70_spike-in_input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYKCJAFXX_n01_ah119_30-70_spike-in_input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5236647
Sequences flagged as poor quality0
Sequence length100
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT103160.19699628407261363TruSeq Adapter, Index 19 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTA99190.18941509710316545TruSeq Adapter, Index 19 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG52450.10015951046537985No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC37500.032.6472350-51
CGATCTC35950.028.3686740-41
TATGCCG43700.028.33758448-49
CGTATGC43950.028.33651546-47
AGTCACG46050.026.78903628-29
AAGGGGG38650.026.330574-75
TCACGTG48300.025.88164930-31
CTCGTAT45400.025.62095844-45
CCGTCTT47900.025.60844252-53
ATCTCGT42950.023.74536342-43
CCAGTCA55050.022.70836326-27
ACGTGAA58050.021.7375132-33
AACGATC47650.021.69932438-39
TGCTTGA57950.021.28954960-61
CGTCTGA60350.020.44085516-17
CTCCAGT64250.019.8957124-25
GTGAAAC63900.019.89493234-35
CACGTCT63750.019.49811214-15
GAAACGA60500.019.38222936-37
CTTCTGC64150.019.37875256-57
Produced by FastQC (version 0.11.2)