FastQCFastQC Report
Wed 7 Mar 2018
HYKCJAFXX_n01_ah119_30-70_spike-in_chip.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYKCJAFXX_n01_ah119_30-70_spike-in_chip.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5218365
Sequences flagged as poor quality0
Sequence length100
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC22050.024.83033650-51
ACGTCCG24050.023.44837232-33
CCGCACA23000.022.98718336-37
TATGCCG25750.021.90074248-49
CGTATGC28100.019.90165546-47
GTCCGCA29700.018.51340534-35
AAGGGGG23500.018.40329274-75
AGTCACG31200.017.92390428-29
CTCGTAT31100.017.68000444-45
CCGTCTT30950.017.61502852-53
GCACATC30100.017.25269138-39
TCACGTC32800.016.90653630-31
ATCTCGT34350.014.912642542-43
AGGGGGG17250.014.30765976-77
CGTCCGC23900.013.567759534-35
CCAGTCA42800.013.34052126-27
ACATCTC38950.013.09106740-41
GTATGCC25300.013.00266548-49
ATGCCGT26000.012.381762550-51
TGCTTGA47350.011.91080360-61
Produced by FastQC (version 0.11.2)