FastQCFastQC Report
Wed 7 Mar 2018
HYKCJAFXX_n01_ah119_25-75_spike-in_input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYKCJAFXX_n01_ah119_25-75_spike-in_input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4271496
Sequences flagged as poor quality0
Sequence length100
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG44210.10350003839404276No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCGA16300.029.697136-37
ACCCGTC20600.025.7792732-33
CCCGATC15550.025.38696538-39
TGCCGTC21400.024.7053250-51
TCACCCG22500.023.60138530-31
CGTATGC23450.022.44537746-47
CGATCTC17200.021.99462740-41
TATGCCG24100.021.93737448-49
CCGTCCC24700.021.59550534-35
CCGTCTT27000.019.4952552-53
AGTCACC28150.019.19810928-29
CTCGTAT24350.018.91424444-45
CCAGTCA30050.018.14053226-27
AAGGGGG21250.017.80854874-75
ATCTCGT24250.015.98836742-43
CGTCTGA34700.015.98016916-17
CACGTCT35300.015.90823614-15
CGTCCCG17700.015.66546136-37
CTCCAGT36900.015.345824-25
CACACGT37000.014.92330412-13
Produced by FastQC (version 0.11.2)