FastQCFastQC Report
Wed 7 Mar 2018
HYKCJAFXX_n01_ah119_25-75_spike-in_chip.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYKCJAFXX_n01_ah119_25-75_spike-in_chip.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6842130
Sequences flagged as poor quality0
Sequence length100
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTA153600.22449149606920651TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC37750.029.19448550-51
CGTATGC45250.025.80827346-47
TATGCCG46850.024.67673748-49
AAGGGGG45000.023.18993474-75
CCGTCTT46650.022.86952452-53
CTCGTAT47450.022.58175744-45
AGGGGGG36750.022.2569376-77
ATGTCAG64100.021.22532734-35
GCCGTCT37450.020.83239252-53
CCAGTCA65650.020.61613726-27
GTATGCC43700.020.4868848-49
TCACATG67500.020.39906330-31
AGTCACA67200.020.38530728-29
ACATGTC68750.020.13114532-33
ATCTCGT53300.020.05933242-43
ATGCCGT45450.018.92309450-51
AAAGGGG44900.018.11168374-75
AGAGCAC114200.018.1066999
CTCCAGT78550.017.29024524-25
TCGTATG49150.017.21076246-47
Produced by FastQC (version 0.11.2)