FastQCFastQC Report
Wed 7 Mar 2018
HYKCJAFXX_n01_ah119_20-80_spike-in_input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYKCJAFXX_n01_ah119_20-80_spike-in_input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6253243
Sequences flagged as poor quality0
Sequence length100
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTA208410.33328306608267105TruSeq Adapter, Index 14 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT125780.20114363059295792TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC52250.033.05333350-51
CCGTATC53650.031.31561938-39
CGTATGC56800.031.10883746-47
TATGCCG57600.030.7582348-49
CTCGTAT59050.029.0485344-45
AAGGGGG49100.027.9577374-75
CCGTCTT61300.027.48443652-53
ATCTCGT60650.027.46849842-43
AGTCACA70200.025.94011528-29
CCAGTCA72150.025.2390326-27
GTATCTC68150.024.859140-41
TTCCGTA73050.024.4788436-37
AGTTCCG74500.024.0021234-35
TCACAGT76500.023.83477830-31
ACAGTTC78200.023.22676532-33
TGCTTGA76750.022.47409860-61
CTCCAGT81100.022.4247424-25
CGTCTGA82350.022.19812816-17
AGAGCAC105300.021.7788899
CACGTCT84550.021.73177714-15
Produced by FastQC (version 0.11.2)