FastQCFastQC Report
Wed 7 Mar 2018
HYKCJAFXX_n01_ah119_20-80_spike-in_chip.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYKCJAFXX_n01_ah119_20-80_spike-in_chip.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5888277
Sequences flagged as poor quality0
Sequence length100
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTA90060.15294796763127821TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC31050.026.0333150-51
TATGCCG35100.023.36350348-49
CGTATGC34800.023.29423346-47
AAGGGGG30700.021.82039574-75
CTCGTAT37200.020.78111844-45
CCGTCTT38100.020.72307852-53
ATCTCGT40250.018.27273842-43
GCCGTCT30200.017.42934252-53
AGGGGGG26100.016.48099176-77
AGTCAAC49600.016.43946634-35
GTATGCC34550.016.25434548-49
ATGCCGT35200.014.88657550-51
AGTCACA56350.014.7192528-29
ACAGTCA57500.014.38473432-33
CCAGTCA60250.014.0004626-27
TCACAGT60950.013.87851130-31
CAATCTC54500.013.79686740-41
TGCTTGA60850.013.70917760-61
TCGTATG39550.013.42673946-47
CTTCTGC62300.013.16379156-57
Produced by FastQC (version 0.11.2)