FastQCFastQC Report
Wed 7 Mar 2018
HYKCJAFXX_n01_ah119_15-85_spike-in_input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYKCJAFXX_n01_ah119_15-85_spike-in_input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5534688
Sequences flagged as poor quality0
Sequence length100
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG167980.30350400962077717TruSeq Adapter, Index 12 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC107530.1942837608913095TruSeq Adapter, Index 12 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG67000.12105470082505103No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG54200.039.9879651
TGCCGTC45000.035.61332748-49
GATCGGA62900.034.5252272
CGTATGC50250.032.40736844-45
AGAGCAC66650.032.2236679
TATGCCG50900.031.99279846-47
TCGGAAG71850.030.5511474
CGTCTGA54250.030.53698316-17
AGTCACC54750.029.87169828-29
CACACGT56450.029.34634812-13
CACGTCT56700.029.09289414-15
CTCGTAT52450.029.03272842-43
CCAGTCA56800.028.95893326-27
AAGGGGG46650.028.81537672-73
CCGTCTT55600.028.65494250-51
CGGAAGA79000.027.5478295
ATCGGAA79650.027.5003073
ATCTCGT54000.027.28575940-41
AGCACAC61400.026.8656710-11
CTCCAGT64750.025.62086724-25
Produced by FastQC (version 0.11.2)