FastQCFastQC Report
Wed 7 Mar 2018
HYKCJAFXX_n01_ah119_15-85_spike-in_chip.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYKCJAFXX_n01_ah119_15-85_spike-in_chip.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5285640
Sequences flagged as poor quality0
Sequence length100
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG54420.10295820373691737No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC24550.023.9280148-49
CGTATGC27700.021.2913844-45
TATGCCG29350.020.25434346-47
CCGTCTT30400.018.7058950-51
AAGGGGG26250.018.35505172-73
CTCGTAT32150.016.66340442-43
ATCTCGT33900.015.38739940-41
AGGGGGG19300.014.73530572-73
CCAGTCA43050.014.57296726-27
AGTCACC43300.014.43449228-29
GCCGTCT22700.013.97416948-49
CAGATCA44700.013.50950934-35
ATGCCGT27200.011.7483246-47
GTATGCC26900.011.70475644-45
TCACCAG55100.011.55642430-31
CTCCAGT53400.011.52842224-25
GATCATC48250.011.49287536-37
TGCTTGA47900.011.3330858-59
CTTCTGC49450.011.26303954-55
CATCTCG25700.011.24568638-39
Produced by FastQC (version 0.11.2)