FastQCFastQC Report
Wed 7 Mar 2018
HYKCJAFXX_n01_ah119_10-90_spike-in_input.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYKCJAFXX_n01_ah119_10-90_spike-in_input.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6917695
Sequences flagged as poor quality0
Sequence length100
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC239330.34596784044396295TruSeq Adapter, Index 6 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG108090.15625146815521643TruSeq Adapter, Index 6 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC56900.032.25162548-49
TCACGCC59350.031.11843930-31
TATGCCG60850.030.4669746-47
CGTATGC61400.030.00401744-45
AGTCACG63100.029.45506528-29
AAGGGGG50850.028.8878372-73
ATCTCGT66000.027.41497840-41
CTCGTAT68200.026.84033442-43
CCGTCTT68450.026.56979650-51
CCAGTCA74700.025.00687426-27
GAGCACA103600.024.7215949
AGAGCAC107050.023.9687658
ACGCCAA78750.023.78125232-33
GCCGTCT54300.023.10757348-49
CGTCTGA82300.022.75454916-17
GATCGGA116650.021.8443681
TGCTTGA83600.021.55954658-59
CACGTCT86800.021.5205814-15
TATCTCG58900.021.42475138-39
GTCACGC59400.021.36093128-29
Produced by FastQC (version 0.11.2)