FastQCFastQC Report
Wed 7 Mar 2018
HYKCJAFXX_n01_ah119_10-90_spike-in_chip.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYKCJAFXX_n01_ah119_10-90_spike-in_chip.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5607019
Sequences flagged as poor quality0
Sequence length100
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG118290.21096771742703208TruSeq Adapter, Index 5 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC89180.15905064705505725TruSeq Adapter, Index 5 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG77410.13805910056662907No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC43200.031.9291648-49
CGTATGC47150.029.0549444-45
TATGCCG48100.028.7249746-47
AAGGGGG43150.027.39655172-73
CCGTCTT49850.026.86870250-51
CTCGTAT46100.024.670742-43
AGTGATC47000.023.84830336-37
AGTCACA60800.023.80645628-29
ACACAGT61050.023.74777632-33
ATCTCGT45450.023.42025840-41
CCAGTCA62800.022.86120826-27
TGATCTC50800.021.69432338-39
TCACACA70850.020.72811130-31
TGCTTGA65900.020.50455758-59
CTTCTGC67100.019.82239354-55
AGGGGGG38200.019.25824774-75
CTCCAGT75150.019.10407424-25
GCCGTCT41500.019.08177650-51
GTATGCC45150.018.52761746-47
ATGCCGT46200.018.25936148-49
Produced by FastQC (version 0.11.2)