FastQCFastQC Report
Fri 7 Jun 2024
HYK33DRX3_n01_Kas-2_rep3_Mock.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYK33DRX3_n01_Kas-2_rep3_Mock.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22459589
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTCTTCGGCCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAA350110.15588441979058476No Hit
CCTCTCTCTTCACAGGCTCACCCTCACAGAAAATCTCTGTGTCTGGAGCA317440.14133829430271408No Hit
GGCCTCTGTGCACAAAGCTGCAAGATCAGCACCGACGTAACCGTGTGTGT292110.13006026067529552No Hit
CCCCTCTTCGGCCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCA238740.10629758184800264No Hit
CCCCACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGT238300.1061016744340246No Hit
CCCGTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGG230160.10247738727543056No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)