FastQCFastQC Report
Mon 20 Mar 2017
HYJKMBGXY_n01_9w-39.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYJKMBGXY_n01_9w-39.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17621760
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG189260.10740130384252197No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG39850.041.11863715
CTCGTAT30700.037.7563844
CACGTCT44200.037.2269314
TCGTATG30950.035.7794645
ACACGTC47250.035.26165813
TATGCCG31850.034.4442748
GTCACAT45400.033.737429
AGTCACA46800.032.94920728
CAGTCAC46850.032.6933327
CGTATGC39750.028.81288146
GTCTGAA60850.027.09867917
CGCTATG49800.026.53102129
CTATAAG60650.025.76636746
GTCGCTA51700.025.42261327
GTATGCC46400.025.05561447
ATGCCGT43400.024.56266249
CTTAAAC44650.024.187581
CACACGT70250.023.86435912
CACATGT65350.023.8603831
TATAAGC65850.023.6796847
Produced by FastQC (version 0.11.2)