FastQCFastQC Report
Mon 20 Mar 2017
HYJKMBGXY_n01_9w-38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYJKMBGXY_n01_9w-38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16540495
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG187130.11313446181628783No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA184640.11162906551466568TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT35800.044.90510644
TCGTATG39450.044.0733845
ACGTCTG43650.041.96323815
TATGCCG42100.040.72581548
CACGTCT47050.039.1501814
CGTATGC49250.035.3031346
ACACGTC53350.034.7206713
AGTCACA50550.034.3934728
CAGTCAC51050.034.12438627
GTATGCC52900.033.32397547
GTCACAG53850.032.35033829
ATGCCGT56100.030.80895249
CACAGTC57250.030.4292831
ACAGTCA57450.030.38284132
GTCTGAA64650.028.65271417
CACACGT71400.025.94324312
TCTCGTA61800.025.84497843
CCGTCTT65950.025.8438752
TCACAGT67200.025.76959430
CTATAAG61400.025.67655646
Produced by FastQC (version 0.11.2)