FastQCFastQC Report
Mon 20 Mar 2017
HYJKMBGXY_n01_9w-35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYJKMBGXY_n01_9w-35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17624359
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG257230.14595140736749632No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG190770.10824223451190482No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC186080.10558114482347983No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT38900.047.35503842
ACGTCTG47400.045.19370315
TCGTATG43250.044.90560543
TATGCCG44400.043.51028446
AGTCACG48550.040.3580328
CACGTCT55900.038.6909414
ACACGTC56700.038.14460813
CAGTCAC54650.036.6737727
CGTATGC53800.035.97213444
GTCACGA54600.035.82304429
GTATGCC55450.035.08827245
ATGCCGT59350.033.01552247
GTCTGAA67700.031.74435617
CACGATC65750.030.22055631
GCCGTCT70600.027.80380849
TGTTACC42300.027.39981313
CGCTATG49750.027.25031729
CCGTCTT73500.027.0829750
CACACGT79750.026.94680212
CTATAAG74900.026.52940446
Produced by FastQC (version 0.11.2)