FastQCFastQC Report
Mon 20 Mar 2017
HYJKMBGXY_n01_9w-34.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYJKMBGXY_n01_9w-34.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16222734
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG233640.14402011399558176No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC180790.11144237463303042No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG173810.10713977064531786No Hit
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGACCCGGCCAGTTAAGGCCAGGAGCGC171470.10569735039728816No Hit
CTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGACCCGGCCAGTTAAGGCCAGGAGCGCATCG163340.10068586466374903No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG37300.046.2434843
CTCGTAT36600.045.80819342
ACGTCTG43650.043.9394315
AGTCACG40350.043.08785628
TATGCCG40400.042.5254946
CACGGCT44800.038.80900231
CACGTCT50000.038.3579414
ACACGTC51600.037.56941613
CAGTCAC49750.035.36266727
GTATGCC50750.034.12429445
ATGCCGT52300.033.17923447
CGTATGC52200.032.8458544
GCTACAT53800.032.57296835
GTCACGG57850.030.888729
GTCTGAA63900.030.39350317
TGTTACC40950.029.22963513
TTACCTC42050.028.54809415
TCTCGTA61800.027.017441
TAGTTGT45850.026.9333389
TCTAATG44300.026.70994620
Produced by FastQC (version 0.11.2)