FastQCFastQC Report
Mon 20 Mar 2017
HYJKMBGXY_n01_9w-32.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYJKMBGXY_n01_9w-32.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16392229
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC199260.12155759903061383No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG45300.043.33144815
CTCGTAT33450.039.0863942
TCGTATG32950.038.2135443
AGTCACT48400.037.41970428
TATCTCG38900.035.6501739
ACACGTC56250.035.3237313
TATGCCG39450.035.0663446
CACGTCT59450.033.248714
CAGTCAC57400.031.49240727
CGTATGC44900.030.27194244
TGTTACC47300.029.90124713
TTACCTC46800.029.63256815
CACACGT67200.029.362712
GTCTGAA69900.028.47700917
CTTATCT53900.027.90479737
GTATGCC51750.027.59809545
TAGTTGT53350.026.574889
TCTAGTT58550.026.571597
CGCTATG42100.026.46713329
GTCGCTA43150.025.82308827
Produced by FastQC (version 0.11.2)