FastQCFastQC Report
Mon 20 Mar 2017
HYJKMBGXY_n01_9w-25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYJKMBGXY_n01_9w-25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16353490
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC163800.10016210607032504No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG37350.044.70252615
CTCGTAT32200.043.38868742
TCGTATG36250.041.4911843
TATGCCG39050.038.6936546
CACGTCT44450.037.5609314
ACACGTC48000.034.92607513
CAGTCAC47050.033.58019327
CGTATGC48600.031.2321444
GTATGCC50000.030.56462345
TGTAATC47250.030.0793736
TAATCTC47850.029.84656738
AGTCACC53700.029.4859928
ATGCCGT52400.029.23028247
GTCTGAA58800.028.86483817
CTTGTAA55200.028.43415534
CGCTATG43450.027.31161329
TTGTAAT56750.027.29307735
CTTAAAC44250.025.9668051
GTCGCTA46400.025.8725127
TGTTACC35300.025.11384413
Produced by FastQC (version 0.11.2)