FastQCFastQC Report
Mon 20 Mar 2017
HYJKMBGXY_n01_9w-22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYJKMBGXY_n01_9w-22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12348615
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC163520.13241970860699762No Hit
GTAAAATCAAAAGACGATGAACAAACTGGGAACACACGAAACAAGGTGGGAGACATCATCGAATCAGAGATGAAC140130.11347831315495706No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCT22900.045.4941439
ACGTCTG32250.036.90250415
CTCGTAT28250.036.0240844
TTCCGTA30250.035.35165836
CACGTCT35300.033.7130914
TCGTATG32550.032.537245
CGTATGC33200.032.21151446
AGTTCCG33950.031.59986934
TATGCCG35000.029.86582248
TATCTCG34650.029.7682841
AGTCACA38800.028.4508528
CGCTATG23300.027.8345529
ACACGTC44900.027.19626613
CAGTCAC41100.027.11073127
GTCACAG41150.026.74256729
GTCGCTA24800.026.70730427
TCTAGTT46450.026.363337
TGTTACC40300.026.27760113
TTACCTC41500.025.51859515
CTTAAAC30750.025.3584671
Produced by FastQC (version 0.11.2)