FastQCFastQC Report
Mon 20 Mar 2017
HYJKMBGXY_n01_9s-47.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYJKMBGXY_n01_9s-47.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15397522
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG261550.16986499515961073No Hit
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGACCCGGCCAGTTAAGGCCAGGAGCGC188560.12246126357215142No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG179610.11664863995648131No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTA156860.10187353523508523No Hit
CTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGACCCGGCCAGTTAAGGCCAGGAGCGCATCG154420.10028886466276847No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG32250.042.35747515
CTCGTAT21750.041.3958842
TCGTATG23600.038.0049443
GTCACAC35150.037.2933529
CACGTCT37200.036.90582314
TATGCCG28200.034.7420246
AGTCACA38400.034.31645228
TCACACT40100.032.6039930
CAGTCAC40800.032.38249227
ACACGTC42800.032.15730713
CGTATGC31150.030.6764244
CACACTT43800.030.00748431
CGCTATG37750.028.60226829
GTCGCTA38750.027.86405427
CTATAAG70400.027.49007646
GTCTGAA52300.026.44905317
GTATGCC37700.026.2619145
TATAAGC75750.025.45736947
ACATCCG77400.025.18724655
ATGCCGT38750.024.12581347
Produced by FastQC (version 0.11.2)