FastQCFastQC Report
Mon 20 Mar 2017
HYJKMBGXY_n01_9s-39.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYJKMBGXY_n01_9s-39.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14241736
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG168080.11801932011659252No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT25800.041.85142
ACGTCTG33550.041.1277515
TCGTATG27350.040.36253743
ACACGTC40300.035.17994313
TATGCCG32550.035.08112746
CACGTCT39650.034.88679514
CGTATGC34500.033.9978744
CAGTCAC42100.030.89171427
GTATGCC40250.029.14113645
AGTCACC48650.026.51978328
ATGCCGT42950.026.42586547
GTCTGAA54250.025.68947617
CACACGT54900.025.38442812
CTATAAG53850.024.79258246
TATAAGC57250.023.3804447
TTACCTC28000.023.16153315
CGCTATG35000.022.96522329
TGTTACC29100.022.87824413
GTCGCTA36400.022.46103327
CCAGTCA59700.022.13115926
Produced by FastQC (version 0.11.2)