FastQCFastQC Report
Mon 20 Mar 2017
HYJKMBGXY_n01_9s-38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYJKMBGXY_n01_9s-38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14818910
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG29600.040.09094615
TCGTATG25950.036.95667343
GTCACAC33100.034.1844329
CTCGTAT22950.033.82079342
TATGCCG29300.033.4383646
CACGTCT35650.033.2862814
ACACGTC40300.030.30132913
AGTCACA39100.028.67405728
ACACAGT40200.027.54525632
CACAGTG40400.027.323633
CAGTCAC41700.027.13422227
CTTAAAC37900.026.2213151
CGCTATG40350.025.99037429
GTCGCTA42350.024.92571327
CACACAG46100.024.69414731
GTCTGAA49650.024.66551217
GTATGCC40050.024.54915645
ACCTATA41000.023.8951456
CGTATGC40750.023.70400244
ATGCCGT43900.022.55345347
Produced by FastQC (version 0.11.2)