FastQCFastQC Report
Mon 20 Mar 2017
HYJKMBGXY_n01_9s-35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYJKMBGXY_n01_9s-35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18481397
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG224330.12138151677603158No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC209600.11341134006265868No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT41150.046.10812842
TCGTATG47550.043.74767343
ACGTCTG51950.042.76384415
TATGCCG50800.040.74579246
CACGTCT58950.037.97732514
CGTATGC55150.037.21936844
AGTCACA56950.036.88886328
CAGTCAC61800.033.99386227
ACACGTC68800.033.2918913
GTATGCC62300.033.16921245
ATGCCGT66850.031.47894147
GTCACAT67800.030.93467929
GTCTGAA79100.028.52184917
TGTTACC46350.028.05759213
TCACGAT83050.027.82997135
CACGATC70000.027.30160336
ATCACGA86200.027.21266234
CCAGTCA82150.026.49694826
CGCTATG44150.026.25308229
CACACGT87950.026.0039912
Produced by FastQC (version 0.11.2)