FastQCFastQC Report
Mon 20 Mar 2017
HYJKMBGXY_n01_9s-34.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYJKMBGXY_n01_9s-34.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12198313
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC149650.12268089857999218No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG29700.042.97486515
ACTTAGG28400.041.2987532
CTCGTAT27150.039.51560642
TCGTATG29600.037.0608443
TATGCCG31250.035.9884846
CACGTCT36000.035.9329314
ACACGTC38300.033.41481413
AGTCACT35550.033.28350428
GTCACTT37450.031.68713429
CAGTCAC37700.031.5686427
TTAGGCA37750.031.526734
TTACCTC34000.030.74205815
CGTATGC36550.030.39198744
GTATGCC39650.028.53796445
TGTTACC37050.028.397213
TAGTTGT38700.027.8993269
TCTAGTT43400.027.7396727
GTCTGAA47150.027.28954717
TACCTCT39400.026.61634316
ATGCCGT42750.026.06602347
Produced by FastQC (version 0.11.2)