FastQCFastQC Report
Mon 20 Mar 2017
HYJKMBGXY_n01_9s-32.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYJKMBGXY_n01_9s-32.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15729032
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG192720.1225250225188683No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG36300.038.8676815
CTCGTAT30150.036.8430642
TCGTATG31200.035.71405843
TATGCCG34400.033.99658246
ACACGTC44550.032.0563113
TATCTCG35750.032.03629339
CACGTCT44250.031.88436914
CGTATGC37100.030.68575344
CAGTCAC46250.028.19369727
CTATAAG57050.025.82059346
CTTAAAC38300.025.7669771
CACCGAT50900.025.75391631
GTATGCC45150.025.74950845
TTACCTC33900.025.54147515
GTCTGAA58950.024.81155617
TATAAGC60050.024.64561747
ATGCCGT46950.024.24787947
TGTTACC36000.024.1467613
AGTCACC55500.023.99221828
CGCTATG39350.023.9327529
Produced by FastQC (version 0.11.2)