FastQCFastQC Report
Mon 20 Mar 2017
HYJKMBGXY_n01_9s-25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYJKMBGXY_n01_9s-25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13520548
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG23000.042.2960843
CTCGTAT20600.041.19536242
ACGTCTG26650.039.4790815
TATGCCG25400.038.7074746
ACTGACC25850.037.49831432
AGTCACT28050.035.0497128
CACGTCT32400.032.89778514
CAGTCAC33300.029.93822727
ACACGTC36450.029.7153213
CGTATGC33900.028.6968844
GTATGCC34900.028.07241645
TGTTACC26450.027.25642813
CGCTATG36450.026.8777729
GTCGCTA38650.025.61561827
ATGCCGT38500.025.4476547
CTTAAAC35000.025.3351021
ACCTATA35100.025.1596896
TTACCTC28800.024.79375515
GTCTGAA43600.024.76454217
CTGACCA40800.024.26514633
Produced by FastQC (version 0.11.2)