FastQCFastQC Report
Mon 20 Mar 2017
HYJKMBGXY_n01_9s-21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYJKMBGXY_n01_9s-21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14568521
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG29400.042.9448915
CTCGTAT26750.042.4286142
TCGTATG28750.040.55739243
GTCACGC29950.038.69989829
AGTCACG30750.037.8052528
TATGCCG31100.036.4949446
ACACGTC36800.034.6831313
TATCTCG34500.033.1976439
CACGTCT38550.033.0194114
CGCCAAT37550.031.69385133
CAGTCAC37400.031.63660227
TCACGCC39100.030.70243530
CACGCCA40400.029.37293831
GCCAATA41050.029.07590734
ATGCCGT40950.028.3903147
CGTATGC40350.028.21408844
GTATGCC41450.027.54838645
GTCTGAA46850.027.4647417
CGCTATG45700.026.26820229
ATATCTC46250.025.06199838
Produced by FastQC (version 0.11.2)