FastQCFastQC Report
Mon 20 Mar 2017
HYJKMBGXY_n01_4w-59.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYJKMBGXY_n01_4w-59.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11425352
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC129560.113396943919102No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG25050.045.1684215
CTCGTAT22600.043.65518642
TCGTATG23200.042.6752443
ACGATAT25450.039.44302435
TATGCCG26100.038.3310746
CACGTCT29950.037.89244514
ACACGTC32000.035.8959913
CCACGAT32300.031.93213533
CGTATGC31750.031.72698644
CAGTCAC32750.031.59994527
TATCTCG31600.031.22106639
CGATATC32350.030.8168736
CACGATA33900.029.61096434
GTATGCC35750.028.8525945
TGTTACC30850.028.73621713
GTCTGAA41400.027.6634617
ATGCCGT36500.027.31446847
CTTAAAC28250.026.7499921
CGCTATG27850.026.63113429
GTCGCTA28700.026.32319327
Produced by FastQC (version 0.11.2)