FastQCFastQC Report
Mon 20 Mar 2017
HYJKMBGXY_n01_4w-53.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYJKMBGXY_n01_4w-53.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14834207
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG192850.13000357889033098No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG38750.042.46388615
CACGTCT43800.037.80344814
CTCGTAT22550.037.3265542
AGTCACA43650.035.56443828
ACACGTC47450.035.040313
CAGTCAC44600.034.96159727
TCGTATG24100.033.20832443
TATGCCG27250.032.6613146
GTCACAT51300.030.12647829
GTCTGAA55600.030.029917
TGTTACC32650.028.63143513
ACATGAG56750.027.59716632
CACACGT62600.026.78051412
TTACCTC35200.026.26426315
CTATAAG57800.026.26063346
CACATGA60300.026.14478731
GTATGCC36250.025.5040245
CGTATGC34100.025.3922544
CGCTATG37000.025.36032929
CCAGTCA63900.024.67192526
Produced by FastQC (version 0.11.2)