FastQCFastQC Report
Mon 20 Mar 2017
HYJKMBGXY_n01_4w-51.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYJKMBGXY_n01_4w-51.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11542273
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC180100.1560351241042384No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATC129630.1123089013749718No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG24900.042.6701415
CTCGTAT22250.040.4670942
TCGTATG24250.038.69444743
TATGCCG25450.037.140946
CACGTCT30100.035.4122414
TGTTACC37000.033.4695913
ACACGTC32550.033.3823713
CGTATGC29250.032.4345444
CGCTATG24150.032.28226529
TTACCTC39150.031.63263315
CAGTCAC31400.030.21160727
TCTAGTT48250.029.4552827
GTCGCTA27200.028.53542927
CTAATGC42250.028.33212921
TTTTTCC72550.027.3948421
TCTAATG44150.027.19122320
TAGTTGT47150.027.0691059
ATGCCGT36600.026.48579247
GTATGCC35900.026.23393645
CTTAAAC29950.026.0372641
Produced by FastQC (version 0.11.2)