FastQCFastQC Report
Mon 20 Mar 2017
HYJKMBGXY_n01_4w-50.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHYJKMBGXY_n01_4w-50.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11669882
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC195380.16742242980691663No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATC132810.11380577798473027No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA125950.10792739806623582TruSeq Adapter, Index 27 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG27250.040.88806515
CTCGTAT24650.039.32565744
TATGCCG26850.037.38890548
TCGTATG28150.035.9068845
TATCTCG28050.035.2963741
TGTTACC40450.033.940613
CGTATGC31150.033.0026346
ACACGTC34500.032.8951413
TTACCTC43050.031.73091315
CACGTCT35600.031.58816514
TCTAGTT51950.029.4152897
TAGTTGT48850.029.3047819
AGTCACA36550.028.7865628
GTATGCC36400.028.7167547
CTCTAGT55650.028.4518136
CAGTCAC37250.027.9674227
CTAATGC48800.027.7100921
TCTAATG49500.027.66679620
TTTTTCC79550.027.627031
ACTCTAG57050.027.572215
Produced by FastQC (version 0.11.2)